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KMID : 1140120110160040371
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Cancer Prevention Research
2011 Volume.16 No. 4 p.371 ~ p.378
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Effects of Cynanchi wilfordii Radix Extracts on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes
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Choi Eun-Ok
Han Min-Ho
Kim Hyang-Suk
Choi Sung-Hee
Choi Yung-Hyun
Kim Byung-Woo
Kim Soo-Yeon
Hwang Hye-Jin
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Abstract
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The present study aimed to evaluate the effects of various extracts of Cynanchi wilfordii Radix on the effects of adipocyte differentiation and adipogenesis using 3T3-L1 preadipocyte cell line. For this study, the extracts of methanol of C. wilfordii (MECWR) was isolated, and then the extracts were fractionated into dichloromethane (CFCWR), ethyl acetate (EAFCWR), butanol (BFCWR) and water (WFCWR) partition layers. The extracts of C. wilfordii showed significant inhibitory activity on adipocyte differentiation in the 3T3-L1 preadipocytes without affecting cell viability as assessed by measuring fat accumulation using Oil Red O staining, and this effect was higher in EAFCWR than in other extracts. In addition, the inhibitory effects of extracts of C. wilfordii were associated with modulation of adipogenic transcription factors such as peroxisome proliferator-activated receptorγ (PPARγ), CCAAT/enhancer binding proteinα (C/EBPα) and C/EBPβ in both translational and transcriptional levels. These results demonstrate that C. wilfordii extracts may be an ideal candidate for obesity relief to disturb adipocyte differentiation and that further studies will be needed for identification of active compounds.
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KEYWORD
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Cynanchi wilfordii radix, Adipocyte differentiation, Adipogenesis, PPARγ, C/EBP
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